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1994-10-25
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Document 2610
DOCN M94A2610
TI Synergistic effect of zidovudine (AZT) and sho-saiko-to (SST) a
traditional kampo drug on HIV replication in lymphocyte cultures.
DT 9412
AU Maitra U; Mohan VP; Klein EB; Inada Y; Lange M; St. Luke's-Roosevelt
Hosp. Ctr., Columbia University, Div. of; Virology, New York, NY 10019.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):265 (abstract no. PB0491). Unique
Identifier : AIDSLINE ICA10/94369965
AB OBJECTIVE: SST is a traditional Kampo (oriental) herbal medicine
demonstrated to have immuno-modulatory effects as well as to inhibit HIV
reverse transcriptase. We tested the effects of AZT and SST singly and
in different combinations on PHA stimulated lymphocyte cultures (LC)
infected in vitro with either HIV or plasma from viremic patients.
METHODS: HIV infected LC were exposed to AZT at 0.5, 1.0 and 3.0 uM AZT
concentration, and to SST at 25, 50 and 100 ug/ml and the combinations
of the two. Supernatants from the cultures were assayed for p24 antigen
on the 7th day and from plasma cultures after 21 days. RESULTS: No
significant reduction of p24 antigen was seen at 1.0uM AZT or 100ug/ml
SST alone whereas an 80 fold reduction occurred at 3.0 uM AZT. Synergy
on combining AZT and SST was observed as follows: AZT at conc'n of 0.5
uM, 1.0 uM and 3.0 uM together with 25 ug/ml of SST reduced p24 antigen
levels to 83, 244 and 732 fold respectively, compared to untreated
cultures. In plasma culture synergy occurred only at AZT conc'n of 1.0
uM or greater on day 21 with a 70 fold reduction in p24 antigen.
CONCLUSIONS: AZT and SST at concentrations pharmacologically achievable
which by themselves are not inhibitory, act synergistically in
inhibiting HIV either in lymphocytes infected with HIV or in plasma
cultures from viremic patients.
DE Cells, Cultured Dose-Response Relationship, Drug Drug Synergism
Drugs, Chinese Herbal/*PHARMACOLOGY Human HIV/*DRUG EFFECTS HIV Core
Protein p24/ANALYSIS Lymphocytes/MICROBIOLOGY Virus Replication/*DRUG
EFFECTS Zidovudine/*PHARMACOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).